About the Series
GenomeWeb and the Association of Biomolecular Resource Facilities are partnering for the second year to produce a series of online seminars highlighting methods, techniques, and instrumentation that support life science research.
Special thanks to the series sponsor, PerkinElmer.
This page will be updated throughout the year as more webinars in the series are scheduled, so please check back regularly!
September 12, 2017 | 1:00 PM ET
Next-Generation CRISPR: New Tools for Efficient Genome Engineering
This webinar will cover recent advances in the use of CRISPR for generating animal models and cell lines.
Our first speaker, CB Gurumurthy of the University of Nebraska Medical Center, will discuss the latest CRISPR strategies and tools available for animal genome editing, with a particular emphasis on strategies for increasing the homology-directed repair mechanism to enable insertion of longer sequences.
While CRISPR has shown very high promise for creating complete knockout (deletion) and subtle genetic changes (e.g., point mutation knock-ins), the generation of complex models, such as long cassette knock-ins and conditional knockout animal models, has remained a major challenge. Dr. Gurumurthy will discuss advances in this area and will present examples of designing knock-in animal models.
Our second speaker, Shondra Miller the Director of the Center for Advanced Genome Engineering at St. Jude Children’s Research Hospital, will share lessons learned from more than 2,000 genome editing projects.
Although the CRISPR/Cas9 genome editing technique is conceptually simple and relatively efficient, creating and identifying correctly targeted clones can be quite laborious. The St. Jude team uses targeted deep sequencing as a tool to help increase the rates of genome editing through more rational design of CRISPR reagents.
Dr. Miller will discuss how her team has used this approach to successfully validate more than 2,000 custom guide RNAs, create more than 400 custom modified cell lines, and create more than 150 custom-modified mouse models.
September 26, 2017 | 1:00 PM ET
CITE-seq: Large-Scale Measurement of Epitopes and Transciptomes in Single Cells
This webinar will describe a protocol and proof-of-principle experiments for Cellular Indexing of Transcriptome and Epitopes by Sequencing (CITE-seq).
CITE-seq combines unbiased genome-wide expression profiling with the measurement of a potentially limitless number of specific protein markers in thousands of single cells.
Marlon Stoeckius, Research Scientist in the Technology Innovation Lab of the New York Genome Center, will present the approach, which first requires conjugating monoclonal antibodies to oligonucleotides containing unique antibody identifier sequences. Next, a cell suspension is labeled with the DNA-barcoded antibodies and single cells are subsequently encapsulated into nanoliter-sized aqueous droplets in a microfluidic apparatus. In each droplet, cells are lysed, antibody and cDNA molecules are indexed with the same unique barcode, and the resulting molecules are converted into libraries. The protein-encoding and RNA-derived libraries can be amplified independently and mixed in appropriate proportions for sequencing in the same lane.
Dr. Stoeckius will discuss proof-of-concept experiments, highlight the method’s compatibility with existing single-cell sequencing approaches, and describe how it can readily scale as the throughput of these methods increases.
October 10, 2017 | 1:00 PM ET
Ask the Experts: Ensuring High- Quality Proteomic Studies with Core Lab Resources
This online, interactive seminar will answer researchers’ most pressing questions about how to gain outstanding research results from proteomic studies
Researchers and clinicians are increasingly adopting proteomics as a go-to method for gaining biological insights and direction. Proteomic core labs are shared resources with the expertise and instrumentation to carry out these specialized studies. Collaboration between researcher and proteomic core combines the expertise and leverages the strengths of both parties. This webinar will provide building blocks on which to jumpstart or strengthen this key relationship.
From study design and sample preparation through data analysis, result interpretation, and overall reproducibility, our panelists will answer your essential questions about setting up and carrying out high-quality proteomic studies. This practical insider info will help researchers and cores to work together and effectively move research forward.
On the registration page for this roundtable webinar, you have the opportunity to submit questions in advance that you would like the webinar panelists to discuss during the live event.
November 14, 2017 | 1:00 PM ET
A Balanced Approach to Assesing Return on Investment for Research Core Facilities
In these times of economic constraint and increasing research costs, shared resource cores have become a cost-effective and essential platform for researchers who seek to investigate complex translational research questions. Cores produce significant value that cannot be captured using traditional financial metrics. Benchmarking studies conducted by the Association of Biomolecular Resource Facilities and other organizations indicate that most research cores do not fully recover operating expenses. As such, these “operational losses” represent institutional investment, which, if well planned and managed, produce future returns for the institution’s research community that extend far beyond subsidized pricing.
Current literature indicates that there is no single measure that can provide an accurate representation of the full picture of the return on research investments.
This presentation attemptsto provide instruction and examples using the Balanced Score Card (BSC), (Kaplan and Norton), as a tool for assessing the return on investment for research core facilities.
The BSC supplements traditional financial measures with criteria to measure performance in three additional areas — customers, internal business processes, and learning and growth. The presenters will also discuss and share their experiences on how they have utilized these ROI approaches to streamline their core operations and make sound investment decisions and strategies to further the mission of their institutions and to meet the expectations of their various investors and key stakeholders.
June 29, 2017 | 2:00 PM ET
Cross-Kingdom Standards in Genomics, Epigenomics, and Metagenomics
This online seminar provides an overview of current standardization efforts in genomics, epigenomics, and metagenomics, with a focus on practical implementation considerations for researchers and labs.
Challenges and biases in preparing, characterizing, and sequencing DNA and RNA can have significant impacts on research in genomics across all kingdoms of life, including experiments in single cells, RNA profiling, and metagenomics. Technical artifacts and contaminations can arise at each point of sample manipulation, extraction, sequencing, and analysis. Thus, the measurement and benchmarking of these potential sources of error are of paramount importance as next-generation sequencing (NGS) projects become more global and ubiquitous.
Fortunately, a variety of methods, standards, and technologies have recently emerged that improve measurements in genomics and sequencing, from the initial input material to the computational pipelines that process and annotate the data.
This webinar reviews work to develop standards and their applications in genomics, including the ABRF-NGS Phase II NGS Study on DNA Sequencing; the FDA’s Sequencing Quality Control Consortium (SEQC2); metagenomics standards efforts (ABRF, ATCC, Zymo, Metaquins), and the Epigenomics QC group of the SEQC2. The webinar also reviews the computational methods for detection, validation, and implementation of these genomic measures.