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A University of Utah team outlines a sequencing strategy designed to assess the capped 5' ends of RNA in formalin-fixed, paraffin-embedded (FFPE) samples. The method — called FFPEcap-seq — includes template switching and an enzymatic enrichment step to boost the representation of 5' RNA ends, the researchers explain, noting that the strategy shares features with existing START-seq and nanoCAGE library construction methods.

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