NEW YORK (GenomeWeb) – Measuring the functional effects of genomic variants in a high-throughput manner has been a challenge for researchers. But in a new study published today in Nature Genetics, a team from the University of California, Los Angeles detailed its efforts to develop a CRISPR-library-based approach for highly efficient and precise genome-wide variant engineering that can be used to profile the effects of large classes of variants in a high-throughput way.

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With the Next Generation Sequencing (NGS), genomes sequencing has been democratized over the last decades with the detection of genomic alterations, thus replacing Sanger sequencing.