NEW YORK (GenomeWeb) – To overcome the challenges of limited phenotyping and bulk-cell resolution that can constrain gene function research performed using DNA barcodes, researchers at the Icahn School of Medicine at Mount Sinai have developed a barcoding system operating at the protein level. And by pairing each protein barcode with a different CRISPR guide RNA, they were able to achieve simultaneous high-dimensional protein-level phenotyping of hundreds of genes with single-cell resolution.

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