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BD Biosciences Clontech, High Throughput Genomics


BD Biosciences Clontech has released its BD Sprint Advantage Single Shots for medium-throughput PCR studies.

According to BD, researchers can “snap-off” individual well strips from the Single Shots’ eight-well strips. Each well contains a lyophilized master mix of BD Tita-nium Taq polymerase, proofreading enzyme, BD TaqStart antibody, dNTPs, and an optimized PCR buffer.

Researchers are required to dissolve the lyophilized PCR reaction mixture using PCR-grade water containing diluted primers and template DNA, and move on to thermal cycling.

High Throughput Genomics has released its Pentadot Plus product for quantifying RNA expression levels.

The tool, based on HTG’s ArrayPlate technology, allows researchers simultaneously to measure RNA expression levels in hundreds of active genes and obtain information about gene expression and protein function, according to HTG.

The Pentadot comprises five oligonucleotides printed at the bottom of each well of a user-programmable 96-well plate. Each well in turn includes one target, three housekeeping genes, and one procedural control that keeps an eye on assay quality.

Researchers can use the Pentadot for applications such as target-gene validation, expression-time courses, treatment profiles, in vitro and in vivo pharmacology, dose-response curves, high-throughput screening, and optimization, metabolism, safety, and diagnostics, HTG said.

Filed under

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