This story was originally published Feb. 2.
RainDance Technologies has launched a product for targeted sequencing of DNA from formalin-fixed paraffin-embedded and fresh frozen tissues samples and has partnered with NuGen Technologies to develop an FFPE sample prep kit, the company said last week.
The new product, called the DeepSeq FFPE Solution, runs on the company's RDT 1000 microdroplet PCR instrument and lets researchers analyze up to 500 genomic targets at up to 50,000-fold coverage per loci, allowing them to discover rare mutations.
The company claims that it provides a “significant enhancement” in allelic sensitivity, compared to other methods that rely on target enrichment and sequencing, as well as to microarray-based methods.
Customers can design their own primer libraries for genomic regions up to 100 kilobases in size. RainDance also offers the ability to integrate next-gen sequencing adapters in the PCR process, and to index samples for multiplexed sequencing.
In a collaboration with NuGen, RainDance is developing a whole-genome amplification FFPE sample prep kit, which will generate microgram amounts of DNA from 100 nanograms of starting FFPE DNA samples.