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NuGen and Caliper to Co-develop Automated Library Construction for Next-Gen Sequencing

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This story was originally published Jan. 27.

NuGen Technologies and Caliper Life Sciences said last week that they will co-develop automated methods for sample prep on Caliper's Sciclone NGS Workstation.

The partners aim to enable automatic processing from a wide range of sample types, such as nucleic acids extracted from sorted cells, fine needle aspirates, formalin-fixed paraffin embedded tissue, and other limited or degraded clinical samples.

The companies will first co-develop a method for RNA-seq library construction from as little as 500 picograms of total RNA, which they expect to be available in March. The method will make use of NuGen's Ovation RNA-seq system and Encore NGS Multiplex System I and will run on Caliper's Sciclone NGS Workstation.

NuGen's CEO Elizabeth Hutt said in a statement that the company's RNA-seq system is the first kit without any requirement for poly-A selection or ribosomal RNA depletion, which "results in a more complete data set and a more comprehensive understanding of the transcriptome, including non-coding RNA."

Aside from RNA-seq library construction, the companies plan to develop automated library construction methods for other applications, including digital gene expression, whole genome, and exome sequencing.

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