This story was originally published Jan. 27.
NuGen Technologies and Caliper Life Sciences said last week that they will co-develop automated methods for sample prep on Caliper's Sciclone NGS Workstation.
The partners aim to enable automatic processing from a wide range of sample types, such as nucleic acids extracted from sorted cells, fine needle aspirates, formalin-fixed paraffin embedded tissue, and other limited or degraded clinical samples.
The companies will first co-develop a method for RNA-seq library construction from as little as 500 picograms of total RNA, which they expect to be available in March. The method will make use of NuGen's Ovation RNA-seq system and Encore NGS Multiplex System I and will run on Caliper's Sciclone NGS Workstation.
NuGen's CEO Elizabeth Hutt said in a statement that the company's RNA-seq system is the first kit without any requirement for poly-A selection or ribosomal RNA depletion, which "results in a more complete data set and a more comprehensive understanding of the transcriptome, including non-coding RNA."
Aside from RNA-seq library construction, the companies plan to develop automated library construction methods for other applications, including digital gene expression, whole genome, and exome sequencing.