NuGen Technologies has launched its Ovation Human FFPE RNA-seq Multiplex System, which provides an end-to-end solution for strand-specific RNA-seq library prep from formalin-fixed paraffin-embedded tissue. The system makes use of NuGen's insert dependent adaptor cleavage technology, which provides targeted depletion of unwanted high abundance transcripts and eliminates the need for additional hybridization based capture steps, according to the company. It also complements NuGen's recently launched Ovation Human Blood RNA-seq Multiplex System.
SoftGenetics' NextGene software version 2.3.4 includes algorithms for detecting copy number variations from exome and targeted sequencing panels, including Life Technologies' Ion AmpliSeq panels. CNVs are detected by comparing the coverage of specified region in a sample project and a control project, and the resulting "coverage ratio" is used to detect CNVs.
Bio-Rad has launched its ddPCR library quantification kit for Life Technologies' Ion Torrent machines. The kit is run on Bio-Rad's QX200 Droplet Digital PCR system and is compatible with the Ion AmpliSeq library kit, and quantifies the Ion AmpliSeq DNA and RNA libraries, maximizes the number of useable reads, enables consistent loading, and optimizes the utilization of every sequencing run, according to the company.