Fluidigm has released its 48.48 Access Array integrated fluidic chip for 454 GS FLX users performing amplicon tagging. The new chip automatically generates emPCR-ready libraries by combining 48 samples and 48 primer sets to produce 48 barcoded samples at a cost of approximately $7 per sample.
The chip can capture up to 12 kilobases of sequence data per sample, or a total of 576 kilobases, according to the company. Upcoming applications, such as long-range PCR, will allow users to target up to 480 kilobases of sequence data per sample, or 23 megabases per array.
The array is Fluidigm's first integrated fluidic chip that allows users to harvest the sample. Once the sample processing has been completed, the chip automatically returns the sample to the inlets, where it can be easily extracted and prepared for sequencing.
DNAStar has launched Lasergene v8.1, its latest sequence analysis software. New features include automated virtual multi-site gateway cloning capability and the ability to simultaneously analyze SNPs from multiple sources.
The software will be able to analyze projects generated from the Roche 454, Illumina, ABI SOLiD and Helicos platforms. It will also be compatible with DNAStar's sequence assembly tool SeqMan NGen and be able to interface with GenVision, the company's data visualization software.
Lasergene v8.1 can be run on Microsoft's Windows XP, Vista, and Windows 7; as well as Mac OS X 10.4, 10.5, and 10.6.
NuGen Technologies has released its Ovation RNA-seq system, a sample-preparation kit for RNA-seq. The kit allows for sample sizes of as low as a few hundred picograms and represents the transcriptome comprehensively, according to the company.
Ovation is the first sample-prep kit that will have no requirements for poly(A) selection or ribosomal RNA depletion, which should reduce bias and give a better representation of the transcriptome, according to NuGen.