Integrated DNA Technologies has released custom primers for the GS FLX Titanium chemistry that it developed under an exclusive agreement with Roche's 454 Life Sciences.
IDT said that its FusionPrimers and Rapid Library Molecular Identification Adaptor Oligos can be used for amplicon and shotgun sequencing, respectively.
The company's design software, SciTools, allows users to create specific primers to target individual or multiple exons from one or more genes.
GS FLX Titanium FusionPrimers have a 25-base pair fixed sequence at the 5' end and enable sequencing of longer amplicons and increased reads per run, the company said. An optional Molecular Identification, or MID, sequence serves as a molecular barcode for multiplexing libraries.
GS FLX Titanium RapidLibrary MID Adaptor Oligos are intended for shotgun sequencing and incorporate MIDs as standard. They are especially useful for sequencing many small samples in parallel and to decrease the sequencing cost per sample, according to IDT.
Complete Genomics has released a new version of Complete Genomics Analysis Tools, open source software that enables users to interpret genomic data.
The updated version includes multi-genome comparison tools, which, according to the company, could allow users to find potential somatic mutations in a tumor-normal comparison, among other applications. It also includes a custom annotation tool to help with data filtering and help customers focus on specific regions of the genome.
Bioo Scientific has released its AIR Genomic and AIR Paired-End DNA Sequencing Kits for the Illumina Genome Analyzer and HiSeq 2000 instruments.
The kits allow researchers to prepare libraries from as little as 50 nanograms of DNA in a single day, according to the company. The new kits expand on Bioo Scientific's previously released sample prep kit for constructing a small RNA sequencing library, the AIR small RNA Sequencing Kit.
The National Center for Biotechnology Information has launched the Database of Genomic Structural Variation. The database will track large-scale genomic variation in both healthy individuals and in disease populations. Additionally, dbVar will collect data on other plant and animal organisms, including those important for agriculture.
The database is part of an international collaboration that includes the Database of Genomic Variants archive at the European Bioinformatics Institute and the Database of Genomic Variants in Toronto. The three centers will exchange information.
GeneGo and Omicsoft have integrated Omicsoft's Array Studio software package for next-generation sequencing analysis with GeneGo's MetaCore data mining suite.
Array Studio provides statistical analysis and visualization tools for both RNA sequencing data and DNA sequencing data. Its alignment algorithms can be applied on the Illumina Genome Analyzer, Roche's 454 GS FLX, and Life Technologies' SOLiD. Integrating those algorithms with GeneGo's MetaCore will enable users to develop hypotheses on biomarkers and drug targets, according to the companies.
The Wellcome Trust Sanger Institute has released version 49 of COSMIC v49, the Catalogue of Somatic Mutations in Cancer. The new version includes updates to 57 curated genes — a candidate gene screen added 1,121 mutations and an additional full-genome screen added 45 coding mutations.