The method, called primer extension capture, or PEC, is especially suited for capturing short DNA fragments in order to sequence a few small genomic regions in large numbers of individuals. Originally developed to analyze areas of interest in the Neandertal nuclear genome, it might also be useful for other types of targeted sequencing applications.
This summer, Agilent plans to introduce a SureSelect kit for paired-end sequencing on the Illumina GA. The company is also working on versions of SureSelect for use with Applied Biosystems' SOLiD sequencer and the 454 Life Sciences platform.
Over the next year or so, Polonator developers plan additional improvements, including reagent kits, increased read lengths, and a simpler library preparation protocol. By 2010, they expect the output per run to increase tenfold, to 100 gigabases.
Life Technologies, J. Craig Venter Institute, BioNanomatrix, Nanosys, Geospiza, HDF Group, Pressure BioSciences, Cofactor Genomics, Ambry Genetics, Affymetrix, Navigenics, Enzymatics, New York State Department of Health, University of Washington
Although the developers note that the protocol particularly benefits the sequencing of AT-rich genomes, they say that it is also quicker than the standard method, which requires PCR amplification, and "should be used routinely to prepare libraries for Genome Analyzer sequencing."
Released this week as a product and a service that uses either catalog or custom arrays, HybSelect initially targets users of the Illumina Genome Analyzer who want to sequence up to 9.5 megabases of genomic DNA.
University of Illinois at Urbana-Champgain, 23andMe, Michael J. Fox Foundaton for Parkinson's Research, Parkinson's Institute and Clinical Center, SeqWright, Roche NimbleGen, Chronix Biomedical, Genome Atlantic, Agilent Technologies
LCS has entered a market varyingly referred to as sequence capture, target enrichment, and sequence enrichment. While the firms playing in this application area cannot agree on a name for it, they all agree that the market has the potential to become, in LCS' words, "very large."