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SeqWright CEO Fei Lu said this week that Lilly is one of an increasing number of pharma customers that have specifically requested sequence capture and sequencing services from the firm.

The method, called primer extension capture, or PEC, is especially suited for capturing short DNA fragments in order to sequence a few small genomic regions in large numbers of individuals. Originally developed to analyze areas of interest in the Neandertal nuclear genome, it might also be useful for other types of targeted sequencing applications.

Agilent Technologies and Febit this week separately announced expansions to their sequence capture product lines, while Roche NimbleGen discussed plans to grow its seq-cap portfolio by year end.

BlueGnome will use the PicoPlex in its SurePlex DNA Amplification system.

This summer, Agilent plans to introduce a SureSelect kit for paired-end sequencing on the Illumina GA. The company is also working on versions of SureSelect for use with Applied Biosystems' SOLiD sequencer and the 454 Life Sciences platform.

Over the next year or so, Polonator developers plan additional improvements, including reagent kits, increased read lengths, and a simpler library preparation protocol. By 2010, they expect the output per run to increase tenfold, to 100 gigabases.

Qiagen licensed HandyLab's extraction technology for use in the sample prep process for its HPV assays.

Short Reads: Mar 24, 2009

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Life Technologies, J. Craig Venter Institute, BioNanomatrix, Nanosys, Geospiza, HDF Group, Pressure BioSciences, Cofactor Genomics, Ambry Genetics, Affymetrix, Navigenics, Enzymatics, New York State Department of Health, University of Washington

The grant will support the development of the firm's pressure cycling technology in sample prep for microbiome studies.

Although the developers note that the protocol particularly benefits the sequencing of AT-rich genomes, they say that it is also quicker than the standard method, which requires PCR amplification, and "should be used routinely to prepare libraries for Genome Analyzer sequencing."

Released this week as a product and a service that uses either catalog or custom arrays, HybSelect initially targets users of the Illumina Genome Analyzer who want to sequence up to 9.5 megabases of genomic DNA.

Short Reads: Mar 17, 2009

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University of Illinois at Urbana-Champgain, 23andMe, Michael J. Fox Foundaton for Parkinson's Research, Parkinson's Institute and Clinical Center, SeqWright, Roche NimbleGen, Chronix Biomedical, Genome Atlantic, Agilent Technologies

SeqWright added Roche NimbleGen's Sequence Capture microarray technology to its offering of services using 454's Genome Sequencer FLX platform.

LCS has entered a market varyingly referred to as sequence capture, target enrichment, and sequence enrichment. While the firms playing in this application area cannot agree on a name for it, they all agree that the market has the potential to become, in LCS' words, "very large."

Febit plans to debut the method at Cambridge Healthtech Institute's Next-Generation Sequencing conference in San Diego next month.

The company continues to develop longer reads for its Genome Sequencer FLX system and to improve its sample-prep process, add more multiplex identifying tags, and add new paired-end library protocols.

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The US has sent its formal notice of withdrawal from the World Health Organization, according to the Wall Street Journal.

Science reports that a draft spending bill would increase the US National Institutes of Health budget by 13 percent.

The Harvard Crimson reports that Harvard and MIT are suing the Department of Homeland Security and ICE over the new international student visa policy.

In Nucleic Acids Research this week: algorithm to determine molecular sequence types and other microbial features, computational method to uncover R-loop structures, and more.