Yale University has been awarded US Patent No. 6,780,599, “Methods of detecting interactions between proteins, peptides, or libraries thereof using fusion proteins.”
Inventors listed on the patent are Andrew Hamilton, Indraneel Ghosh, and Lynne Regan.
According to its abstract, the patent protects assays using fusion proteins with GFP fragments and test polypeptides for investigating protein-protein interactions.
Tibotec has been awarded US Patent No. 6,800,437, “Method of monitoring a biological system by labeling with an apo metal binding protein.”
Inventors listed on the patent are Inge Dierynck, Christiaan Roelant, Rudi Pauwels, Koenraad Van Acker, and Jan De Kerpel.
According to its abstract, the patent describes reporter molecules and tags that may be used to monitor a target substance in a biological system. Specifically, the invention relates to the use of an apo metal binding protein as a reporter molecule or tag, the abstract states. The apo metal binding protein may be bound to a protein or tissue, or introduced into a cell. The patent also describes methods of utilizing the apo metal binding protein for detecting a cell expressing a protein of interest, localizing a protein in a cell, and designing a therapeutic agent for treating a disease or infection, the abstract states.
Applera has been awarded US Patent No. 6,800,445, “Systems for sensitive detection of G-protein coupled receptor and orphan receptor function using reporter enzyme mutant complementation.”
Inventors listed on the patent are Michelle Palmer, Melissa Gee, Bonnie Tillotson, and Xiao-jia Chang.
According to its abstract, the patent describes methods for detecting G-protein coupled receptor (GPCR) activity; methods for assaying GPCR activity; and methods for screening for GPCR ligands, G-protein-coupled receptor kinase (GRK) activity, and compounds that interact with components of the GPCR regulatory process. Included are methods for expanding ICAST (Intra-cistronic Complementation Analysis Screening Technology) for assaying GPCR activity with applications for ligand fishing, and agonist or antagonist screening, the abstract states. Included are also more specific methods for using ICAST complementary enzyme fragments to monitor GPCR homo- and hetero-dimerization with applications for drug lead discovery, and ligand and function discovery for orphan GPCRs, the abstract states.