Thermo Fisher Scientific this week introduced NUNC cell culture inserts, now available with polycarbonate membranes, the company said.
The new inserts are appropriate for cell-interaction and cultivation studies in areas such as tissue engineering, co-cultivation, invasion, chemotaxis, and transport, and do not require preparation with matrix coating.
NUNC cell culture inserts with polycarbonate membranes are available in four sizes, in 24-, 12-, or 6-well multi-dish configurations, and in 0.4, 3.0, and 8.0 µm pore sizes.
Packed in a multi-dish configuration, the NUNC polycarbonate cell culture inserts have a sterility assurance level of 10.6, and are endotoxin-free and surface treated for optimal cell attachment.
In independent trials, the inserts have been shown to support cell lines including BHK, MDCK, CACO-2, HeLa, WRC, Hep-G2, HEp-2, L9299, CHO, LLC-PK, and 3T3.
According to the manufacturer, the membranes can be removed from the multi-well, multi-dish design, and can be used for sectioning in transmission electron microscopy studies when using a glass knife. The company said the polycarbonate membranes have larger pore sizes that make them appropriate for invasion and chemotaxis studies.
Beckman Coulter has introduced the gTox flow kit, a flow-cytometric approach to genotoxicity testing, the company announced this week.
The gTox flow assay enumerates micronucleated red cells in the peripheral blood of treated mice. It is appropriate for preclinical drug testing, environmental testing, and other academic and commercial toxicology applications, the company said.
It is also one of a battery of tests that chemical companies can use to comply with Registration, Evaluation, Authorization, and Restriction of Chemical Substances regulations in Europe, Beckman said.
The gTox flow kit was developed for the Beckman Coulter FC 500 flow cytometer in both single- or dual-laser excitation configurations. The kit incorporates a proprietary RBC permeation method and can be performed at room temperature without centrifugation preparation.
The gTox flow assay can be performed manually or on Beckman Coulter’s Biomek NXP laboratory automation workstation. According to the manufacturer, the gTox flow assay yields first results in less than two hours.
Clontech has released its Protein-DNA Binding Assay, the company announced this week.
The assay uses sensitive, quantitative, chemiluminescent ProLabel detection, and can be performed in a 96-well plate, which the manufacturer said eliminates the need for radioactive labeling and gel electrophoresis.
A small (~6 kDa) ProLabel tag is fused to the protein of interest to generate a fusion protein that is capable of producing a strong chemiluminescent signal via an enzyme complementation assay. The ProLabel tag allows direct detection of specific binding between the protein of interest and a biotinylated dsDNA oligonucleotide.
The biotin moiety on the oligonucleotide permits the resulting protein-DNA complex to be captured on the 96-well plate, which is coated with streptavidin.