Tecan and pION have launched the PAMPA Explorer, a new manual system for drug permeability screening using the double-sink parallel artificial membrane permeability assay, or PAMPA.
Tecan is supplying its new Infinite M200 plate reader for the system. pION has commercialized PAMPA and developed applications that are highly correlated with Caco-2, gastrointestinal tract, and blood-brain barrier permeation.
The PAMPA Explorer is a manual, upgradeable entry-level system based on the Infinite M200 reader, which is capable of screening hundreds of compounds per day. The quad4 monochromators technology in the Infinite M200 allows users to select any wavelength from UV to NIR, and to perform absorbance, excitation, and emission scans, the companies said.
PAMPA Explorer can be upgraded to a Tecan Freedom EVO platform system when sample throughput increases, Tecan said.
AnaSpec this week introduced its SensoLyte Homogeneous AFC Caspase-8 assay kit.
The kit uses Ac-IETD-AFC (7-amido-4-trifluoromethylcoumarin) as the fluorogenic indicator to measure caspase-8 activity. Upon cleavage of the substrate by caspase-8, free AFC emits a yellow-green fluorescence, which can be quantified at excitation/emission of 380nm/500nm.
A bi-functional assay buffer in this kit is optimized for cell lysis and measurement of the enzyme activity. This kit can assay caspase-8 activity in cells grown in a 96-well plate or in larger plates or flasks. The kit is adaptable for high throughput screening of apoptosis inducers and inhibitors.