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Recent Cell-Based Assay Papers of Note: Jul 21, 2006

Recent Cell-Based Assay Papers of Note
Journal Title Lead Institution(s) Synopsis
Analytical Biochemistry
2006 Jun 9;
[Epub ahead of print]
A miniaturized cell-based fluorescence resonance energy transfer assay for insulin-receptor activation Merck, North Wales, Penn.; and Vertex Pharmaceuticals, San Diego Describes the development of a miniaturized, ratiometric, cell-based assay for identifying small-molecule insulin mimetics and potentiators
Assay and Drug Development Technologies
2006 Jun; 4(3): 273-84
Validated high-throughput screening of drug-like small molecules for inhibitors of ErbB2 transcription Buck Institute for Age Research, Novato, Calif. Describes a whole-cell, high-throughput screening assay using a luciferase reporter to identify lead compounds capable of cell permeability and selective silencing of ErbB2 transcription
Assay and Drug Development Technologies
2006 Jun; 4(3): 253-62
Development of the high-throughput screening assay for identification of agonists of an orphan nuclear receptor Merck Research Laboratories, West Point, Penn. Describes a homogenous cell-based resonance energy transfer assay to identify RNA agonists using ß-lactamase as the reporter gene
Assay and Drug Development Technology
2006 Jun; 4(3): 263-72
A homogeneous cell-based assay to measure nuclear translocation using beta-galactosidase enzyme fragment complementation DiscoveRx, Fremont, Calif. Exemplifies the use of DiscoveRx's EFC technology to measure nuclear translocation of the glucocorticoid receptor in CHO-K1 cells
Biomedical Microdevices
2006 Jun 27;
[Epub ahead of print]
Microplate cell-retaining methodology for high-content analysis of individual non-adherent unanchored cells in a population Bar-Ilan University, Ramat Gan, Israel Describes the Microtiter plate Cell Retainer, which enables high-content, time-dependent analysis of the same single non-adherent and non-anchored cells in a large cell population
Journal of Biomolecular Screening
2006 Jun; 11(4): 423-34
Identification of RAS-mitogen-activated protein kinase signaling pathway modulators in an ERF1 Redistribution screen BioImage, Copenhagen, Denmark Describes a cell-based, high-throughput screen using an ERF1 Redistribution assay to identify compounds modulating the RAS-MAPK signaling pathway
Journal of Biomolecular Screening
2006 Jun; 11(4): 377-89
Miniaturization and validation of a sensitive multiparametric cell-based assay for the concomitant detection of microtubule-destabilizing and microtubule-stabilizing agents Université Joseph Fourier, Grenoble, France Describes a 96-well cell-based assay that uses double-immunofluorescence staining for identifying microtubule-destabilizing and microtubule-stabilizing agents
Journal of Biomolecular Screening
2006 Jun; 11(4): 369-76
Cell-based screening and validation of human novel genes associated with cell viability Chinese National Human Genome Center, Beijing Describes a 96-well cell-based assay that relies on the down-regulation of Renilla luciferase activity to identify novel human genes associated with cell viability
Journal of Biomolecular Screening
2006 Jun; 11(4): 351-8
A novel cell-based assay for G-protein coupled receptor-mediated cyclic adenosine monophosphate response element binding protein phosphorylation Neurocrine Biosciences, San Diego Describes a high-throughput method of quantifying CREB phosphorylation using an Odyssey infrared imaging system
Journal of Biomolecular Screening
2006 July 14;
[Epub ahead of print]
Multiparametric cell cycle analysis by automated microscopy Nerviano Medical Sciences, Nerviano, Italy Describes the use of a Cellomics ArrayScan to develop a four-color muliparametric high-content analysis approach for cell-cycle analysis of adherent cells based on detection of DNA content and cell cycle markers
Journal of Immunological Methods
2006 Jun 19;
[Epub ahead of print]
A high-throughput electrochemiluminescent cell-binding assay for therapeutic anti-CD20 antibody selection Genentech, South San Francisco, Calif. Describes the development of a cell-binding assay that uses high-binding capacity carbon electrode plates for rapid attachment of WIL2 cells, and electrochemiluminescence for detection
Molecular Biotechnology
2006 Jun; 33(2): 141-8
Identification of effective siRNA blocking the expression of SARS viral envelope E and RDRP genes Shanghai Institute of Brain Functional Genomics, East China Normal University, China Describes the development of a cell-based assay to screen siRNAs to block expression of two genes of the SARAS virus
2006 Jun; 12(6): 1129-41
Identification of RNA-binding proteins that regulate FGFR2 splicing through the use of sensitive and specific dual-color fluorescence minigene assays University of Pennsylvania School of Medicine, Philadelphia Describes the development of a series of fluorescent splicing reporter minigenes to establish cell-based screens for identifying splicing regulatory proteins