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Recent Cell-Based Assay Papers of Note: Jul 21, 2006

Recent Cell-Based Assay Papers of Note
Journal Title Lead Institution(s) Synopsis
Analytical Biochemistry
2006 Jun 9;
[Epub ahead of print]
A miniaturized cell-based fluorescence resonance energy transfer assay for insulin-receptor activation Merck, North Wales, Penn.; and Vertex Pharmaceuticals, San Diego Describes the development of a miniaturized, ratiometric, cell-based assay for identifying small-molecule insulin mimetics and potentiators
Assay and Drug Development Technologies
2006 Jun; 4(3): 273-84
Validated high-throughput screening of drug-like small molecules for inhibitors of ErbB2 transcription Buck Institute for Age Research, Novato, Calif. Describes a whole-cell, high-throughput screening assay using a luciferase reporter to identify lead compounds capable of cell permeability and selective silencing of ErbB2 transcription
Assay and Drug Development Technologies
2006 Jun; 4(3): 253-62
Development of the high-throughput screening assay for identification of agonists of an orphan nuclear receptor Merck Research Laboratories, West Point, Penn. Describes a homogenous cell-based resonance energy transfer assay to identify RNA agonists using ß-lactamase as the reporter gene
Assay and Drug Development Technology
2006 Jun; 4(3): 263-72
A homogeneous cell-based assay to measure nuclear translocation using beta-galactosidase enzyme fragment complementation DiscoveRx, Fremont, Calif. Exemplifies the use of DiscoveRx's EFC technology to measure nuclear translocation of the glucocorticoid receptor in CHO-K1 cells
Biomedical Microdevices
2006 Jun 27;
[Epub ahead of print]
Microplate cell-retaining methodology for high-content analysis of individual non-adherent unanchored cells in a population Bar-Ilan University, Ramat Gan, Israel Describes the Microtiter plate Cell Retainer, which enables high-content, time-dependent analysis of the same single non-adherent and non-anchored cells in a large cell population
Journal of Biomolecular Screening
2006 Jun; 11(4): 423-34
Identification of RAS-mitogen-activated protein kinase signaling pathway modulators in an ERF1 Redistribution screen BioImage, Copenhagen, Denmark Describes a cell-based, high-throughput screen using an ERF1 Redistribution assay to identify compounds modulating the RAS-MAPK signaling pathway
Journal of Biomolecular Screening
2006 Jun; 11(4): 377-89
Miniaturization and validation of a sensitive multiparametric cell-based assay for the concomitant detection of microtubule-destabilizing and microtubule-stabilizing agents Université Joseph Fourier, Grenoble, France Describes a 96-well cell-based assay that uses double-immunofluorescence staining for identifying microtubule-destabilizing and microtubule-stabilizing agents
Journal of Biomolecular Screening
2006 Jun; 11(4): 369-76
Cell-based screening and validation of human novel genes associated with cell viability Chinese National Human Genome Center, Beijing Describes a 96-well cell-based assay that relies on the down-regulation of Renilla luciferase activity to identify novel human genes associated with cell viability
Journal of Biomolecular Screening
2006 Jun; 11(4): 351-8
A novel cell-based assay for G-protein coupled receptor-mediated cyclic adenosine monophosphate response element binding protein phosphorylation Neurocrine Biosciences, San Diego Describes a high-throughput method of quantifying CREB phosphorylation using an Odyssey infrared imaging system
Journal of Biomolecular Screening
2006 July 14;
[Epub ahead of print]
Multiparametric cell cycle analysis by automated microscopy Nerviano Medical Sciences, Nerviano, Italy Describes the use of a Cellomics ArrayScan to develop a four-color muliparametric high-content analysis approach for cell-cycle analysis of adherent cells based on detection of DNA content and cell cycle markers
Journal of Immunological Methods
2006 Jun 19;
[Epub ahead of print]
A high-throughput electrochemiluminescent cell-binding assay for therapeutic anti-CD20 antibody selection Genentech, South San Francisco, Calif. Describes the development of a cell-binding assay that uses high-binding capacity carbon electrode plates for rapid attachment of WIL2 cells, and electrochemiluminescence for detection
Molecular Biotechnology
2006 Jun; 33(2): 141-8
Identification of effective siRNA blocking the expression of SARS viral envelope E and RDRP genes Shanghai Institute of Brain Functional Genomics, East China Normal University, China Describes the development of a cell-based assay to screen siRNAs to block expression of two genes of the SARAS virus
2006 Jun; 12(6): 1129-41
Identification of RNA-binding proteins that regulate FGFR2 splicing through the use of sensitive and specific dual-color fluorescence minigene assays University of Pennsylvania School of Medicine, Philadelphia Describes the development of a series of fluorescent splicing reporter minigenes to establish cell-based screens for identifying splicing regulatory proteins
The Scan

Genetic Tests Lead to Potential Prognostic Variants in Dutch Children With Dilated Cardiomyopathy

Researchers in Circulation: Genomic and Precision Medicine found that the presence of pathogenic or likely pathogenic variants was linked to increased risk of death and poorer outcomes in children with pediatric dilated cardiomyopathy.

Fragile X Syndrome Mutations Found With Comprehensive Testing Method

Researchers in Clinical Chemistry found fragile X syndrome expansions and other FMR1 mutations with ties to the intellectual disability condition using a long-range PCR and long-read sequencing approach.

Team Presents Strategy for Speedy Species Detection in Metagenomic Sequence Data

A computational approach presented in PLOS Computational Biology produced fewer false-positive species identifications in simulated and authentic metagenomic sequences.

Genetic Risk Factors for Hypertension Can Help Identify Those at Risk for Cardiovascular Disease

Genetically predicted high blood pressure risk is also associated with increased cardiovascular disease risk, a new JAMA Cardiology study says.