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Recent Cell-Based Assay Papers of Note: Apr 21, 2006

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Recent Cell-Based Assay Papers of Note
Journal Title Lead Institution(s) Synopsis
Archives of Toxicology
2006 Apr 6;
[Epub ahead of print].
High concordance of drug-induced human hepatotoxicity with in vitro cytotoxicity measured in a novel cell-based model using high-content screening Pfizer Global Research and Development, Sandwich, UK Describes the use of high-content screening methods to develop and validate an in vitro, cell-based model to assess the potential for drug-induced human hepatotoxicity.
Biosensors and Bioelectronics 2006 Apr 15;
21(10): 1893-9.
High-speed detection of circulating tumor cells Scripps-PARC Institute for Advanced Biomedical Science, Palo Alto, Calif. Describes development of an instrument that uses fiber-optic array scanning technology to detect rare cells in circulation.
Biotechnology and Bioengineering
2006 Mar 28;
[Epub ahead of print].
Parallel, high-throughput purification of recombinant antibodies for in vivo cell assays Cambridge Antibody Technology, Cambridge, UK Discusses a method for high-throughput, parallel purification of secreted proteins to analyze large numbers of proteins in cell-based assays for the discovery of protein therapeutics
Cytometry Part A
2006 Mar; 69(3): 200-2.
Evaluation of a high-content screening fluorescence-based assay analyzing the pharmacological modulation of lipid homeostasis in human macrophages University of Regensburg, Germany Describes a tool that uses high-content fluorescence imaging to evaluate new agents that may induce cholesterol efflux.
IEEE Transactions on Biomedical Engineering
2006 Apr; 53(4): 762-6.
Automated segmentation, classification, and tracking of cancer cell nuclei in time-lapse microscopy HCNR Center for Bioinformatics, Harvard Medical School, Boston Presents an automated system and image-analysis method to segment, classify, and track individual cells in a living population over the course of a few days.
Nature Methods
2006 May; 3(5)
[Epub ahead of print].
Fluorescent cell barcoding in flow cytometry allows high-throughput drug screening and signaling profiling Stanford University, California Describes a cell-based multiplexing technique called fluorescent cell barcoding that can analyze single cells in a high-content multi-parameter setting.
Nature Methods
2006 May; 3(5)
[Epub ahead of print].
High-throughput RNAi screening by time-lapse imaging of live human cells European Molecular Biology Laboratory, Heidelberg, Germany Details an automated platform for high-content RNAi screening that uses time-lapse fluorescence microscopy of live HeLa cells expressing histone-GFP to report on chromosome segregation and structure.
Nature Reviews Drug Discovery
2006 Apr;
5(4): 343-56.
Cellular imaging in drug discovery Serono Pharmaceutical Research Institute, Plan-les-Ouates, Switzerland Review paper describes how cellular imaging technologies contribute to the drug-discovery process.
Photochemistry and Photobiology
2006 Mar-Apr; 82(2): 351-8.
Photoconvertible fluorescent protein EosFP: biophysical properties and cell biology applications University of Ulm, Germany Presents the spectroscopic properties of a UV-stimulated green-to-red fluorescent protein called EosFP that can be used in a variety of cellular imaging, developmental biology, and high-content screening applications.
Proceedings of the National Academy of Sciences
2006 Mar 7; 103(10): 3775-80.
A small interfering RNA screen for modulators of tumor cell motility identifies MAP4K4 as a pro-migratory kinase Genomics Institute of the Novartis Research Foundation, San Diego Describes a genomic screen using siRNA and an automated microcopy-enabled wound-healing assay to identify inhibitors of cell migration.
Proceedings of the National Academy of Sciences
2006 Mar 7; 103(10): 3698-703.
Functional mapping of disease susceptibility loci using cell biology Columbia Genome Center, Columbia University, New York Demonstrates a functional cell-based RNAi screen in combination with statistical genetic results to identify candidate genes for disease susceptibility.
Proceedings of the National Academy of Sciences
2006 Mar 21; 103(12): 4445-50
Automated local bright feature image analysis of nuclear protein distribution identifies changes in tissue phenotype Lawrence Berkeley National Laboratory, Berkeley, Calif. Describes imaging methods to quantify the distribution of fluorescently stained nuclear protein NuMA in different mammary phenotypes obtained using 3D cell culture.
RNA
2006 Mar 23;
[Epub ahead of print].
Identification of inhibitors of ribozyme self-cleavage in mammalian cells via high-throughput screening of chemical libraries Harvard Medical School and Children's Hospital, Boston Describes the development of a high-throughput, luciferase-based cellular assay to identify small molecules that can inhibit ribozyme self-cleavage.
Science
2006 Apr 14; 312(5771): 217-24.
The fluorescent toolbox for assessing protein location and function National Center for Microscopy and Imaging Research, University of California, San Diego Review article discusses the characteristic benefits and limitations of fluorescent probes to study proteins, with emphasis on protein detection in live versus fixed cells.
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