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Recent Cell-Based Assay Papers of Note
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| Journal | Title | Lead Institution(s) | Synopsis |
| Archives of Toxicology 2006 Apr 6; [Epub ahead of print]. |
High concordance of drug-induced human hepatotoxicity with in vitro cytotoxicity measured in a novel cell-based model using high-content screening | Pfizer Global Research and Development, Sandwich, UK | Describes the use of high-content screening methods to develop and validate an in vitro, cell-based model to assess the potential for drug-induced human hepatotoxicity. |
| Biosensors and Bioelectronics 2006 Apr 15; 21(10): 1893-9. |
High-speed detection of circulating tumor cells | Scripps-PARC Institute for Advanced Biomedical Science, Palo Alto, Calif. | Describes development of an instrument that uses fiber-optic array scanning technology to detect rare cells in circulation. |
| Biotechnology and Bioengineering 2006 Mar 28; [Epub ahead of print]. |
Parallel, high-throughput purification of recombinant antibodies for in vivo cell assays | Cambridge Antibody Technology, Cambridge, UK | Discusses a method for high-throughput, parallel purification of secreted proteins to analyze large numbers of proteins in cell-based assays for the discovery of protein therapeutics |
| Cytometry Part A 2006 Mar; 69(3): 200-2. |
Evaluation of a high-content screening fluorescence-based assay analyzing the pharmacological modulation of lipid homeostasis in human macrophages | University of Regensburg, Germany | Describes a tool that uses high-content fluorescence imaging to evaluate new agents that may induce cholesterol efflux. |
| IEEE Transactions on Biomedical Engineering 2006 Apr; 53(4): 762-6. |
Automated segmentation, classification, and tracking of cancer cell nuclei in time-lapse microscopy | HCNR Center for Bioinformatics, Harvard Medical School, Boston | Presents an automated system and image-analysis method to segment, classify, and track individual cells in a living population over the course of a few days. |
| Nature Methods 2006 May; 3(5) [Epub ahead of print]. |
Fluorescent cell barcoding in flow cytometry allows high-throughput drug screening and signaling profiling | Stanford University, California | Describes a cell-based multiplexing technique called fluorescent cell barcoding that can analyze single cells in a high-content multi-parameter setting. |
| Nature Methods 2006 May; 3(5) [Epub ahead of print]. |
High-throughput RNAi screening by time-lapse imaging of live human cells | European Molecular Biology Laboratory, Heidelberg, Germany | Details an automated platform for high-content RNAi screening that uses time-lapse fluorescence microscopy of live HeLa cells expressing histone-GFP to report on chromosome segregation and structure. |
| Nature Reviews Drug Discovery 2006 Apr; 5(4): 343-56. |
Cellular imaging in drug discovery | Serono Pharmaceutical Research Institute, Plan-les-Ouates, Switzerland | Review paper describes how cellular imaging technologies contribute to the drug-discovery process. |
| Photochemistry and Photobiology 2006 Mar-Apr; 82(2): 351-8. |
Photoconvertible fluorescent protein EosFP: biophysical properties and cell biology applications | University of Ulm, Germany | Presents the spectroscopic properties of a UV-stimulated green-to-red fluorescent protein called EosFP that can be used in a variety of cellular imaging, developmental biology, and high-content screening applications. |
| Proceedings of the National Academy of Sciences 2006 Mar 7; 103(10): 3775-80. |
A small interfering RNA screen for modulators of tumor cell motility identifies MAP4K4 as a pro-migratory kinase | Genomics Institute of the Novartis Research Foundation, San Diego | Describes a genomic screen using siRNA and an automated microcopy-enabled wound-healing assay to identify inhibitors of cell migration. |
| Proceedings of the National Academy of Sciences 2006 Mar 7; 103(10): 3698-703. |
Functional mapping of disease susceptibility loci using cell biology | Columbia Genome Center, Columbia University, New York | Demonstrates a functional cell-based RNAi screen in combination with statistical genetic results to identify candidate genes for disease susceptibility. |
| Proceedings of the National Academy of Sciences 2006 Mar 21; 103(12): 4445-50 |
Automated local bright feature image analysis of nuclear protein distribution identifies changes in tissue phenotype | Lawrence Berkeley National Laboratory, Berkeley, Calif. | Describes imaging methods to quantify the distribution of fluorescently stained nuclear protein NuMA in different mammary phenotypes obtained using 3D cell culture. |
| RNA 2006 Mar 23; [Epub ahead of print]. |
Identification of inhibitors of ribozyme self-cleavage in mammalian cells via high-throughput screening of chemical libraries | Harvard Medical School and Children's Hospital, Boston | Describes the development of a high-throughput, luciferase-based cellular assay to identify small molecules that can inhibit ribozyme self-cleavage. |
| Science 2006 Apr 14; 312(5771): 217-24. |
The fluorescent toolbox for assessing protein location and function | National Center for Microscopy and Imaging Research, University of California, San Diego | Review article discusses the characteristic benefits and limitations of fluorescent probes to study proteins, with emphasis on protein detection in live versus fixed cells. |
Recent Cell-Based Assay Papers of Note: Apr 21, 2006
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