Porvair Sciences has introduced a gas-permeable Rayon microplate sealing film, the company announced recently.
Manufactured from high-permeability, medical grade Rayon, the new heat sealing film provides a uniform air and CO2 exchange with all microplate wells. The breathable membrane also reduces evaporation, enhancing longer-term incubation experiments, according to the company.
The new sealing film allows direct sample recovery with single or multichannel pipettors and robotic probes. Designed for use with both manual and automated heat sealers, the new sealing film uses a non-cytotoxic adhesive to minimize sample cross-contamination. Though available sterile, the new-gas permeable Rayon microplate sealing film cannot be autoclaved without reducing permeability, the company said.
Platypus Technologies announced this week that it has added the Oris cell invasion assay kit, 96-well format, to its line of cell-based assays.
The Oris Invasion Assay kit is supplied with an Oris BME coated 96-well plate, Oris BME solution, Oris Cell Seeding Stoppers, Oris Stopper Tool, and Oris Detection Mask. Unlike invasion assays using BME-coated, cell culture membrane inserts, the Oris Invasion Assay does not require the cells to pass through a membrane.
The Oris Cell Seeding Stoppers create a 2-mm detection zone where adherent cells cannot invade until after the stoppers are removed. Seeded cells are overlaid with BME to create a 3D matrix. This creates a more physiologically relevant environment for cell invasion and allows for real-time monitoring of cells producing true invadopodia into the detection zone, the company said.
This assay allows researchers to view cells invading into a 3D basement membrane extract in real time by using a microscope or digital imaging system. Invasion can be quantitated in the same well by staining cells and using a plate reader.
Amaxa has introduced four new Human Stem Cell Nucleofector Kits, for single reactions and up to 96-well transfections, suitable for various stem cell lines. The company said that with these kits, transfection efficiencies of 60 percent up to 80 percent, in addition to high viability, can be achieved for stem cells.
Amaxa's Stem Cell Nuclefoector Kits enable researchers to transfect to embryonic stem cells, as well as other types of stem cells, including mesenchymal, hematopoietic, or neural stem cells, without compromising their potential for differentiation, according to the company.
Invitrogen this week announced the availability of a proprietary test kit to screen compounds for cardiotoxicity earlier in the drug discovery process.
The company said that the Predictor hERG Fluorescence Polarization Assay Kit is the first commercially available non-radioactive, homogenous assay kit for the high-throughput screening of compounds interacting with the hERG ion channel.