Platypus Technologies this week announced the addition of the Oris Universal cell migration assembly kit, 96-well format, to its product line of cell-based assays. Oris cell seeding stoppers replace membrane-based, cell culture inserts to generate real-time results in chemokinesis or 2D closure studies.
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Unlike the original Oris cell migration kits, the Oris Universal assembly kit allows scientists to coat the 96-well plate with any extracellular matrix component to create a migration or invasion assay. A 2D closure assay can be designed by leaving the plate uncoated.
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The Oris cell seeding stoppers are then inserted into each well, where the stopper tip creates a protected 2-mm round zone in the center of each well. Cells are seeded, allowed to adhere, and then the stoppers are removed, exposing a detection zone into which cells can move.
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An overlay of an ECM on the cells after stopper removal provides a 3D environment for studying invasion. Cell movement can be monitored in real time by staining and viewing cells with a microscope or digital imaging system. A detection mask is snapped to the bottom of the plate, permitting quantitation of stained cells using a plate reader.
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The assay is offered as a 1-plate starter pack and as a 5-plate multi-pack. A collagen I coated plate version of the Oris assembly kit is also available.