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Nikon, Trevigen

Nikon’s Ti series inverted microscopes can now capture images at two wavelengths simultaneously through the use of dual cameras, the company announced this week.
The stratum structure, which uses Nikon’s 200 mm infinity space, allows an extra camera to be added via the optional back port in conjunction with a camera on the side imaging port, according to the manufacturer.
The rear camera can be individually centered and focused to guarantee registration of both images simultaneously with no pixel shift. The company said that with two cameras, it is possible to observe rapid calcium reactions with dual emission ratiometric dyes such as Indo-1, without dividing the CCD chip and compromising image resolution.
A dual camera configuration also permits researchers to compare high S/N ratio images when observing interactions between fluorescent proteins with fluorescence resonance energy transfer. Each FRET channel can be separated by wavelength and sent to separate cameras.
Extra components, such as laser tweezers and a photo activation unit, can also be mounted simultaneously on upper and lower tiers of the new microscope. A two-tier epi-fluorescent filter turret holding up to a total of 10 filter cubes can also be incorporated, and is appropriate for multi-user facilities and researchers working with different dyes and fluorescent proteins, the company said. In addition, each motorized filter wheel can be controlled separately.

Trevigen has launched its Cultrex 24-well cell-invasion assay kits to study invasive properties of cells. The kits are designed to accelerate screening of compounds that influence cellular invasion through extracellular matrices, the company said.
To provide a platform for the analysis of responses to chemokines, toxins, drugs, and other analytes of interest for larger numbers of cells per well, Trevigen also debuted its Cultrex 24-well BME cell-invasion assay kit. “The modular format of [the assay] offers flexible utility, and sufficient insert size for informative results,” Trevigen said.
The company said it also sells a Cultrex 96-well BME assay for researchers with higher-throughput needs.
Trevigen said it created Cultrex 24-well cell-invasion assay kits to “accelerate the screening process for compounds that influence cellular invasion through extracellular matrices.” This process is “fundamental to angiogenesis, embryonic development, immune responses, tumor cell metastasis, and cancer research,” the firm said.

The assays use a simplified Boyden chamber design with an 8 µm polycarbonate membrane. Detection of cell migration is quantified using Calcein AM, which is internalized by the cells; and intracellular esterases to cleave the acetomethylester moiety, according to Trevigen.


The firm said that free Calcein fluoresces brightly, which “may be used to quantitate the number of cells that have migrated using a standard curve.”

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