Interferometric Technique Adds Third Dimension to PALM Microscopy | GenomeWeb

Scientists at the National Institutes of Health and the Janelia Farm Research Center campus of the Howard Hughes Medical Institute have developed a new imaging technology that they claim produces the best three-dimensional resolution of cells with an optical microscope.

The technique, called interferometric photoactivated localization microscopy, or iPALM, can locate fluorescent labels in images within 10 nm to 20 nm, a Janelia Farm researcher told CBA News this week.

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