I-STEM to use Velocity 11’s HTS platform
Velocity11, a provider of automation solutions based in Menlo Park, Calif., announced this week that it has installed its high-throughput automated stem cell culture and screening platform at I-STEM in Evry, France.
I-STEM is a new organization formed by INSERM, the French national institute of health, and AFM, the French muscular dystrophy association, to validate the feasibility of using human embryonic stem cells for therapeutics. The main objective of I-STEM will be to create the French Center for Stem Cell Research.
According to Veocity11, its flexible, robotic platform marks the first time that stem cell culture and screening will be automated to this level of complexity in a 96-well microplate format and at this level of throughput.
Using a design based on the BioCel 1800 Automation System, the I-STEM platform will use Velocity11's VWorks Automation Control software to manage protocols, and execute process runs. Liquid handling on the new I-STEM platform will be handled by two VPrep Pipetting Systems.
The first VPrep System will use a 96LT pipetting head (volume range 2 to 200 µl) and will be dedicated to stem cell culturing. The second VPrep System will employ a 96 ST head (volume range 0.5 to 30µl) and will be dedicated to compound management. The I-STEM platform will store and dispense microplates and tip racks using a compact VStack Automated Plate Stacker. The Velocity11 Lid Hotel will be used to handle microplate lids.
The Velocity11 Bar Code Label Print and Apply Station, VCode, will be used for plate tracking. A PlateLoc Thermal Plate Sealer has been integrated into the I-STEM platform in order to provide the capability to seal compound plates after each run.
Velocity11A designed a custom air filtration system to adhere to I-STEM's stringent requirements for operational sterility. To avoid any risk of contamination, the BioCel 1800 Automation System enclosure was equipped with a class100 ULPA filter. Four extraction funnels have been installed, one on each corner of the BioCel System deck.