Guava Technologies has launched its Guava Nexin assay, the company announced this week.
The newly revised assay, for the first time, provides all necessary reagent components in a single cocktail, the company said. The newly reformulated Guava Nexin reagent eliminates the need for pre-assay dilutions, cocktail preparations, or time consuming wash steps. The assay protocol is identical for use on all Guava systems, using both tube- and plate-based approaches.
The new mix-and-read Guava Nexin assay relies on a two-dye strategy. The assay uses Annexin V-PE to detect the translocation of phosphatidylserine to the external surface of the membrane of apoptotic cells. The assay incorporates this PS-Annexin V-PE binding as an indicator of early-stage apoptotic cells. The cell impermeant dye 7-AAD is included in the Guava Nexin reagent as an indicator of membrane structural integrity.
7-AAD is excluded from live healthy cells and early apoptotic cells, but permeates late stage apoptotic and dead cells. In conjunction, the two dyes, added in one step as a reagent cocktail, provide quick and accurate determination of early- and late-stage cellular apoptosis, the company said.