The panel provides multiplexed HCS analysis of 11 human cytotoxicity parameters over three time points (acute at 30 minutes, early at 24 hours, and chronic at 72 hours), generating 10-point dose-response curves in duplicate for up to 16 test compounds and four control toxins.
GE Healthcare, Promega, Applied Biophysics, PerkinElmer Life, Analytical Sciences, Epitome Biosystems, Amaxa Biosystems, Millipore, Cellumen
GE Healthcare this week announced the release of IN Cell Investigator 1.3 software for high content analysis and screening.
The software combines Spotfire DecisionSite visual analytics software with the IN Cell Developer toolbox and validated IN Cell Analysis modules.
IN Cell Investigator 1.3 uses multi-processor computing to speed up overall analysis and the cell-tracking feature allows scientists to measure changes in cellular movements in response to treatment.
The subpopulation analysis tools enable classification of cells in different applications, such as apoptosis or cell cycle analysis. Classification and cell tracking can be combined to monitor phenotypic changes in individual cells over time, which can be used in RNAi screens or calcium imaging.
Promega this week announced the launch of its ONE-Glo luciferase assay reagent.
The company said that the product is based on a luciferase substrate developed by Promega scientists that renders the reagent more tolerant to different sample and reaction components.
ONE-Glo exhibits reduced influence from luciferase inhibitors, which results in fewer false positive readings, according to the manufacturer.
The reagent is also optimized for batch and continuous-process handling.
The extended bright light output of ONE-Glo allows for high sensitivity, especially when extended incubation is required prior to reading results.
ONE-Glo is immediately available through the Promega Early Access Program and will be commercially available at the end of August.
Applied Biophysics last week released Electric Cell-Substrate Impedance Sensing, an automated method to monitor cell behavior under flow conditions.
The ECIS measurement non-invasively follows the impedance of cell-coated electrodes in real time without the use of fluorescence or radio-labeled materials. Cells are grown in disposable flow arrays, where they are cultured on the floor of a flow channel that is 400 µm high and 0.5 cm wide.
Cell behavior and its response to changing flow conditions are monitored by eight independent measuring electrodes located along the 5 cm length of the channel. The flow array can be used with all ECIS models and fits into the standard array holder.
To support this new flow array, Applied BioPhysics offers a complete flow module designed to interface with both existing and new ECIS 1600R and ECIS1600/800 systems. This turn-key module includes a peristaltic pump designed to operate within the high humidity of a tissue culture incubator along with medium reservoirs, flow equalizer, tubing, fittings, and a start-up supply of flow arrays.
The system comes with new software integrated within the standard ECIS menus. This application allows researchers to program flow conditions, both continuous and pulsed, that are automatically implemented during the course of an experiment.
Flow rates may be called up that range from those used to simply perfuse cells to those that subject cells to the highest shear stresses encountered in vivo. Upon analysis, users may call up graphs of flow conditions that are correlated with ECIS time course impedance data for easy interpretation.
PerkinElmer Life and Analytical Sciences this week announced a new introductory configuration for its Opera high-content screening system called Opera LX.
Opera LX can be custom configured with a laser combination of choice, and can be equipped with options such as an environmental control unit, lenses, UV light source including a second camera for parallel image acquisition, liquid handling, plate handling/storage, and incubation.
All accessory units can be field-upgraded, which enables a user to start with a basic configuration for assay development and small-to-medium throughput campaigns that can be gradually upgraded to the Opera QEHS system.
Epitome Biosystems this week announced the commercial launch of its Ti-Tyr phosphorylation profiling chip.
Ti-Tyr chips use Epitome's proprietary EpiTag technology to assess cell activity states by profiling tyrosine phosphorylation.
First generation Ti-Tyr profiling chips are being offered through an early-access product program beginning with sample analysis performed at Epitome with subsequent customized technology transfer for in-house use.
Amaxa Biosystems has updated its cell database to include support for its new transfection products 96-well Shuttle and HiFect as well as for new cell categories.
More transfection data is being entered on a daily basis, according to the company.
Millipore this week announced the availability of its Neurite Outgrowth Assay kit.
The kit uses high-quality, validated, target-specific detection reagents for profiling in a variety of species, including human, mouse, and rat. Large-scale screening is simplified through the assay reagents 24-hour stability at room temperature.
The kit’s primary antibody is immunofluorescence-based to specifically label neurites and neuronal cell bodies in heterogeneous cell populations.
Millipore also this week announced the availability of the CellCiphr cytotoxicity profiling assay kit using human HepG2 cells.
The panel uses human HepG2 cells in an assay comprising validated, multiplexed detection reagents and protocols for testing pre-clinical compounds against well-recognized cytotoxicity parameters.
The assay was developed in collaboration with Cellumen and their proprietary cellular systems biology approach to investigating cytotoxicity.