Cold Spring Harbor Laboratory Press this week said that its current issue of Cold Spring Harbor Protocols addresses live-cell imaging techniques.
The first protocol, available here, details a microinjection procedure to inject fluorescently labeled proteins into individual cells, CSHLP said.
The second protocol, available here, outlines the construction and use of an enclosed microscope chamber. The device is designed to optimize the cell-culture environment for microscopic viewing.
Both protocols are freely accessible, CSHLP said.
Invitrogen this week announced the availability of OptiCHO Express kits for the growth and transfection of dihydrofolate reductase-deficient Chinese hamster ovary DG44 cells in suspension culture. The kit provides a complete workflow protocol for developing stable cell lines and includes all of the necessary components for expression vector construction, high-efficiency transfection, and recombinant protein expression in a serum-free environment, Invitrogen said.