A number of new products were introduced at the Society for Biomolecular Sciences annual conference in Seattle last week. Following is a summary of new products relating to cell-based assays, cellular analysis, and high-content screening.
Cisbio HTRF assays
Cisbio launched three HTRF cell-based assays related to targets in inflammation and metabolic disease. The first assay quantifies cGMP to assess the activation of screening targets such as guanylyl cyclases or cGMP-regulated phosphodiesterases.
The second assay quantifies prostaglandin E2, an essential inflammation mediator in cell supernatants or whole cells. The third assay measures cortisol levels in liver microsomes, whole cells, and animal serum.
All of Cisbio’s HTRF assays are based on homogenous fluorescence readouts and can be miniaturized to 1,536-well formats.
The Epic is a high-throughput, label-free screening platform based on resonant waveguide optical sensing. It performs biochemical and cell-based assays for drug-discovery applications. The system comprises two main components: a disposable 384-well microplate with integrated biosensors, and a high-throughput screening-compatible microplate reader that can read as many as 40,000 wells in eight hours.
For more information on the Corning Epic, see the 11/28/2005 issue of CBA News.
Molecular Devices FlexStation 3
Molecular Devices launched the FlexStation 3 multi-detection benchtop microplate reader. FlexStation 3 is based on the company’s SpectraMax M5 multi-detection system with added liquid handling. The platform addresses a wide variety of assays that require liquid handling, including fast kinetic assays such as calcium mobilization or dual luciferase reporter gene assays; as well as traditional endpoint or slow kinetic assays requiring automated liquid handling prior to plate reading, MDCC said. The system uses an 8- or 16-channel pipettor head to transfer reagents from 96 or 384 wells of a source plate to a read plate.
See related story, this issue.
PerkinElmer JANUS Cellular Workstation, AlphaScreen SureFire assays, and LANCE Ultra assays
The JANUS Cellular Workstation is a fully automated liquid-handling platform designed specifically for medium- and high-throughput cell-based assays. The station combines reagent chemistries, detection systems, and liquid handling for walk-away automation, the company said.
The AlphaScreen SureFire assays combine technology from PerkinElmer and TGR Biosciences. The assay enables rapid screening of previously difficult kinase drug targets through homogenous non-radioactive measurement of phosphorylated full-length proteins, PerkinElmer said.
LANCE Ultra is an improved homogenous time-resolved fluorescence platform for TR-FRET assays.
See related story, this issue.
Sophion QPatch HT
Sophion Bioscience launched the QPatch HT, a 48-channel gigaseal patch clamp system that can be used to measure the activity of both voltage-gated and ligand-gated ion channels in live cells.
The system includes 48 individual low-noise patch clamp amplifiers and pressure controllers, and eight pipettes attached to a fluid-handling robot to keep pace with the liquid-handling demand of up to 48 simultaneous patch clamp experiments. The 48-channel QPlate measurement plate builds on the same technology as the company’s original 16-channel QPlate.
TTP Labtech Acumen eX3
TTP Labtech launched the Acumen eX3 microplate scanning cytometer. The Acumen uses three laser lines (405 nm, 488 nm, and 633 nm) to excite 96-, 384-, or 1,536-well plates containing cell samples, and detects resulting fluorescence signals from labels using four photomultiplier tube detectors, for a maximum of 12 channels of data.
For more on the Acumen eX3, see the 9/22/06 issue of CBA News.