Cadus Technologies has been awarded US Patent 7,361,498, “Yeast cells expressing modified G proteins and methods of use therefore.”
The inventors listed on the patent are Dana Fowlkes, James Broach, John Manfredi, Jeremy Paul, Joshua Trueheart, Christine Klein, and Andrew Murphy.
According to its abstract, the patent pertains to novel yeast cells that are useful for the expression of heterologous G protein coupled receptors. The yeast cells can be used in assays to screen for modulators of GPCRs. Specifically, the patent describes novel yeast cells that express a heterologous GPCR and mutant and/or chimeric G protein subunit molecules that functionally integrate the heterologous GPCR into the pheromone signaling pathway of the yeast cell. The patent also provides for the expression of heterologous GPCRs that are functionally integrated into the yeast cell membrane using a yeast α-factor leader sequence. Drug discovery assays using these yeast cells are also described.