BresGen has been awarded US Patent 7,432,104, “Methods for the culture of human embryonic stem cells on human feeder cells.”
The inventors listed on the patent are Maisam Mitalipova and Ian Lyons.
According to its abstract, the patent describes methods and cell culture medium for the generation of human pluripotent embryonic stem cells. Human embryonic stem cells are cultured with human granulosa feeder cells, muscle cells, fallopian ductal epithelial cells, bone marrow stromal cells, and skin fibroblasts, and the embryonic stem cells maintain their pluripotent phenotype. The human pluripotent embryonic stem cells can be cultured without feeder cells, and in the presence of supplemental growth factors. They can alternatively be cultured with conditioned medium obtained from a cell culture capable of maintaining human embryonic stem cells in a pluripotent state.