Beckman Coulter this week announced two additions to its line of cell signal-transduction reagents—Phospho-MAPKAPK-2 and Phospho-STAT3 kinase antibodies. These single-color flow cytometric reagents measure kinase activation in the cytoplasm.
According to the manufacturer, the Phospho-MAPKAPK2 marker is particularly useful in the study of pro-inflammatory mediator release, actin reorganization, and cell invasion mechanisms. The Phospho-STAT3 marker that translocates to the cell nucleus upon activation plays a role in many cellular processes such as cell growth and apoptosis, the company said.
SVision this week announced the launch of its live cell image analysis software, SVCell 1.0. According to the company, the live cell module extends the capability of the “teachable” SVCell software to address time-lapse microscopy applications.
The software automatically detects and characterizes cells or subcellular objects in time-lapse images. It defines boundaries for biological objects over time for dynamic spatial-temporal analyses, and also quantifies the kinetic gain or loss of fluorescence. It provides kinetic detection, measurements, and classifications within an image and data analysis interface that links overlays, images, data charts and tables together in a single frame.
SVCell 1.0 can be used to quantitatively characterize dynamic events and phenotypes in time-lapse multi-channel fluorescence, phase contrast, and brightfield images. The software is suitable for a broad range of applications, including kinetic studies and label-free applications.
Cold Spring Harbor Press this month released Cold Spring Harbor Protocols, an online resource of methods for marking molecules to identify gene alterations and metabolic shifts. It is structured to be interactive, with each protocol cross-linked to related methods, information panels, and illustrative material.
The first method describes how to grow cells from bacteria, yeast, insects, or mammals in media containing the stable isotope nitrogen-15. The second method describes how to label DNA probes with fluorescent markers of six different colors, which span the visible spectrum. These labeled DNA probes can be used simultaneously in fluorescent in situ hybridization experiments, during which the probes bind to specific chromosomal regions of interest. The chromosomes can then be viewed under a microscope.
Molecular Devices last month announced the launch of its live cell Neurotransmitter Transporter Uptake Assay Kit. According to the company, researchers will now have a single tool to screen for live-cell kinetic uptake of dopamine, norepinephrine, and serotonin. The kit contains a homogeneous, fluorescence-based, high-throughput screening assay that eliminates the use of radioactive tags or labels.
The assay can be used in both kinetic and endpoint modes and is performed in 96- or 384-well formatted microplates.