BD Biosciences last week announced the availability of new ZsGreen fluorescent protein vectors. Two varieties of the vectors — pIRES2-ZsGreen1 or pZsGreen1-1 — are available for cellular assays.
According to BD Biosciences, the pIRES2-ZsGreen1 vector contains an internal ribosome entry site, which permits both the cloned gene and the ZsGreen1-encoding region to be translated from a single mRNA.
The pZsGreen1-1 is a promoterless vector for studying transcriptional regulation, BD Biosciences said.
Promega said recently that it has introduced its pGL4 luciferase reporter vectors.
According to the company, the reporter gene vectors are optimized for expression in mammalian cells, and come in numerous configurations, including those with the synthetic luc2 (Photinus pyralis) and hRluc (Renilla reniformis) genes.
Both the reporter genes and the vector backbone have been engineered to reduce the number of consensus transcription factor binding sites, reducing background and the risk of anomalous transcription, Promega said.