Applied Biosystems has been awarded US Patent 7,368,296, “Solid phases optimized for chemiluminescent detection.”
The inventors listed on the patentare Brooks Edwards, Timothy Geiser, Steven Menchen, Alison Sparks, and John Voyta.
The patent describes solid supports for chemiluminescent assays, according to its abstract. The solid support includes multiple probes covalently or physically attached to the support surface and a chemiluminescent-enhancing moiety incorporated onto the surface or into the bulk of the support. The solid support can be a multi-layered support including an upper probe binding layer (e.g., an azlactone polymer layer or porous functional polyamide layer) adjacent to a cationic microgel layer. The azlactone-functional polymer can be a copolymer of dimethylacrylamide and vinylazlactone crosslinked with ethylenediamine. The cationic microgel layer can be a cross-linked quaternary onium salt containing polymer.
The patent also provides a method and a kit for conducting chemiluminescent assays using the solid supports. The kit comprises a dioxetane substrate, a biopolymer probe-enzyme complex, and a solid support. The solid support can be an azlactone functional polymer layer adjacent to a cationic microgel layer; a porous polyamide functional layer adjacent to a cationic microgel layer; or a quaternized azlactone functional polymer layer.
The University of Texas System has been awarded US Patent 7,368,232, “High-throughput assay for virus entry and drug screening.”
The inventors listed on the patent are Robert Davey and Andrey Kolokoltsov.
According to its abstract, the patent provides a rapid virus entry/binding detection assay. An enzyme such as luciferase, incorporated at the C-terminal end of viral envelope proteins of the HIV Nef protein, specifically associates with cell membranes to deliver the enzyme into viral particles upon viral assembly. Virus entry/binding can then be assayed by determining the enzymatic activities in infected cells. The assay allows high-throughput, non-radioactive detection of virus entry within 30 minutes of virus-cell contact.
This assay provides high signal-to-noise ratio and can be used to screen compounds that affect virus-cell binding and entry. The design also permits packaging of potential therapeutic proteins into functional virus particles and delivering them to specific cellular targets.
Invitrogen has been awarded US Patent 7,368,086, “Functionalized fluorescent nanocrystals, and methods for their preparation and use.”
The inventor listed on the patent is Imad Naasani.
According to its abstract, the patent covers functionalized fluorescent nanocrystal compositions and methods for making and using these compositions. The compositions are fluorescent nanocrystals coated with at least one material. The coating material has chemical compounds or ligands with functional groups or moieties with conjugated electrons and moieties that make the fluorescent nanocrystals soluble in aqueous solutions. The coating material makes these functionalized fluorescent nanocrystal compositions water soluble, chemically stable, and able to emit light with a high quantum yield and/or luminescence efficiency when excited with light. The coating material may also have chemical compounds or ligands with moieties for bonding to target molecules and cells in addition to moieties for cross-linking the coating. In the presence of reagents suitable for reacting to form capping layers, the compounds in the coating may form a capping layer on the fluorescent nanocrystal with the coating compounds operably bonded to the capping layer.