Amaxa this week released one Nucleofector kit and two 96-well Shuttle kits designed to transfect mouse dendritic cells.
All three kits are dedicated to the transfection of DNA and siRNAs. According to the manufacturer, both 96-well Shuttle kits are optimized to work with low cell numbers (down to 2.5x104 mature cells per well of a 96-well Nucleocuvette).
Applied Biosystems this week announced the availability of its Silencer Select line of synthetic siRNAs.
Silencer Select siRNAs have been developed using a new proprietary siRNA design algorithm to more reliably silence specific genes and chemical modifications to enhance the specificity of the silencing effect, the company said.
Invitrogen this week introduced its CHO CD EfficientFeed kit for identifying the optimum feed regimen for any fed-batch CHO-based cell culture.
The CD EfficientFeed kit comes with 1 liter each of CHO CD EfficientFeed A and CHO CD EfficientFeed B feed supplements and a manual.
Thermo Fisher Scientific this week introduced its Nalgene 2L Bio Bottle, a two-liter centrifuge bottle designed to support high-volume sample processing.
When used with the Thermo Scientific Sorvall RC12BP centrifuge and H-12000 swinging-bucket rotor, Nalgene 2L Bio Bottles enable scientists to process 12 liters of sample in a single run.
The Nalgene 2L Bio Bottle and sealing cap are leak-proof up to 7,333 x G and have an operating temperature range from 4° C to 22° C, with storage to 0° C. Constructed of chemical-resistant polypropylene copolymer, the bottles meet USP Class VI standards and are certified non-cytotoxic and non-hemolytic. The bottles are autoclavable.
Beckman Coulter this week introduced two new IOTest reagents — Anti-NTB-A-PE and CD226 (DNAM-1)-PE. Both are monoclonal antibodies to cell surface receptors for natural killer cell studies.
These reagents are compatible with Beckman Coulter's FC 500 series and Cell Lab Quanta SC systems as well as other instrument platforms.
According to Beckman Coulter, these two new antibodies complete the company's NK-targeted mAb family. The pre-formulated, standardized IOTest Anti-NTB-A-PE and CD226 (DNAM-1)-PE antibodies are provided in 50-test vials and are labeled for research use only.
The IOTest Anti-NTB-A-PE reacts with a member of a newly discovered family of NK receptors called signaling lymphocyte activation molecules (or SLAM), which are important functional receptors in lymphocytes and other leukocytes. In certain cases of NTB-A expression, downstream mechanisms are thought to be involved in the induction of T cell co-stimulation and the regulation of B cell tolerance.
IOTest CD226 (DNAM-1)-PE recognizes a putative cell-corruption surveillance receptor in NK and cytotoxic T lymphocyte cells. It may also play a role in transendothelial cell migration.