NEW YORK — Researchers have developed a CRISPR-based, single-cell lineage tracer method and used it to study how lung cancer metastasizes, with the goal of predicting metastatic behavior.
Phylogenetic trees based on the genomes of tumors and metastases can trace back mutation patterns that emerge during cancer development. By combining Cas9-based lineage tracing and single-cell RNA sequencing, researchers from the University of California, San Francisco, and elsewhere have now developed a way to prospectively track cancer progression in a mouse model.