Qiagen QIAprep& Viral RNA UM Kit
Qiagen has launched the QIAprep& Viral RNA UM Kit to simplify and accelerate PCR analysis in epidemiological studies of RNA viruses, including SARS-CoV-2. The new kit combines a two-minute, liquid-based sample preparation step with real-time PCR detection in a workflow that can be automated with standard lab equipment for any throughput, assay, and reaction need for both single-plex and multiplex testing. Qiagen said the workflow comprises taking an aliquot from a nasopharyngeal, oropharyngeal, or nasal swab in transport media; adding the aliquot to a buffer optimized for viral RNA template preparation without degradation; and combining this mixture with RT-qPCR reaction mix for subsequent real-time PCR on any thermal cycler using any assay. The entire workflow takes less than an hour compared to about three hours for standard extraction-based qPCR processes, and can be used to process up to 2,600 samples per eight-hour shift per thermal cycler, the company said.
Fabric Genomics Fabric GEM Algorithm for Genetic Disease Diagnosis
Fabric Genomics has launched Fabric GEM, an algorithm designed to identify the likely genetic cause of rare diseases from next-generation sequencing data. Fabric GEM, the latest product in the Fabric Enterprise software suite, uses artificial intelligence to analyze sequencing data (including complex structural variants) and patient clinical information in order to perform probabilistic disease matching and speed genetic diagnoses. The algorithm was developed in collaboration with Mark Yandell of the University of Utah and Stephen Kingsmore of Rady Children's Institute for Genomic Medicine, Fabric said.
Lexogen Luthor 3' mRNA-seq Library Prep Kit
Lexogen has launched the Luthor 3' mRNA-seq library prep kit for ultra-low input and single-cell sequencing. At sequencing depth of 1 million reads, the kit allows detection of up to 15,000 genes from one cell and up to 13,000 genes from 10 picograms of purified RNA input. Using T7 high-resolution original RNA (THOR) amplification, the kit amplifies RNA directly, without a cDNA intermediate.
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