Shoba Anantha at Bitesize Bio says that the most effective way the reduce amplicon contamination in labs running PCR is to "get the bleach out." She references a 1992 study by Andrus and Prince that reports a dilution of household bleach as more effective at destroying DNA detectable by PCR than 2.0 N HCl. Anantha suggests that a 10 to 100x dilution of commercial bleach, such as Clorox, should be used to wipe down every surface in the lab. "Generously spray workstations/equipment/pipettes with 10 percent bleach, then let it sit for 15-30 minutes (coffee break time!). Then wipe up the bleach and follow up with a water rinse and wipe," she says, adding that "this procedure should be carried [out] before and after each PCR, and definitely after a spill. I religiously stick to a weekly cleaning routine of all of my PCR equipment and workspace and that has gone a long way to preventing amplicon contamination."
Whiter Whites and Abated Amplicon Contamination
Apr 09, 2010