At Bitesize Bio, blogger Suzanne gives some tips for getting lots of pure double-stranded DNA from a gel extraction. Her 10 suggestions include trimming off as much of the agarose gel as possible and minimizing the DNA's exposure to UV light or, instead, using methylene blue or crystal violet stains. For commercial silica spin kits, she advises heating the elution buffer and using the good ethanol.
When in Doubt, Do a Wash
Nov 12, 2007