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What's on the Inside

Bala at Bitesize Bio posts a protocol that is even faster than colony PCR to detect cloned inserts. This "quick and dirty" method, called colony cracking, begins by lysing a colony and running the extract on a gel. The clones are then identified based on their mobility. Plasmids with inserts, being bigger, will travel more slowly than plasmids without them. "Obviously, the larger the insert, the easier it will be to distinguish between positive and negative clones, but researchers claim to be able to detect inserts of only 200bp," writes Bala.

The Scan

Gone, But Now Reconstructed SARS-CoV-2 Genomes

In a preprint, a researcher describes his recovery of viral sequences that had been removed from a common database.

Rare Heart Inflammation Warning

The Food and Drug Administration is adding a warning about links between a rare inflammatory heart condition and two SARS-CoV-2 vaccines, Reuters reports.

Sandwich Sampling

The New York Times sent tuna sandwiches for PCR analysis.

Nature Papers Describe Gut Viruses, New Format for Storing Quantitative Genomic Data, More

In Nature this week: catalog of DNA viruses of the human gut microbiome, new dense depth data dump format to store quantitative genomic data, and more.