What's on the Inside | GenomeWeb

What's on the Inside

Bala at Bitesize Bio posts a protocol that is even faster than colony PCR to detect cloned inserts. This "quick and dirty" method, called colony cracking, begins by lysing a colony and running the extract on a gel. The clones are then identified based on their mobility. Plasmids with inserts, being bigger, will travel more slowly than plasmids without them.

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