Skip to main content
Premium Trial:

Request an Annual Quote

This Week in PNAS: Oct 9, 2012

An international team led by investigators at the French National Institute for Agricultural Research, the US Department of Energy's Joint Genome Institute, and the Wellcome Trust Centre for Human Genetics presents results from a genome sequencing study of the button mushroom, Agaricus bisporus, in the early, online issue of the Proceedings of the National Academy of Sciences. The team generated draft genome sequences for two A. bisporus representatives — a cultivated European strain from the A. bisporus variety bisporus and a California strain from the burnetti variety that makes it home in the leaf litter around woody plants. By folding in transcriptome data for different mushroom developmental stages and for mushrooms grown on different substrates, the group uncovered clues about the fungal adaptations that the mushroom uses for growth in humic acid-rich environments, including genes coding for enzymes involved in the decomposition of leaves and other plant materials.

Adult stress levels and socio-economic status during childhood appear to influence some DNA methylation differences that exist between individuals within a community, according to a study by researchers at the University of British Columbia, Simon Fraser University, and Stanford University. The researchers performed array-based DNA methylation profiling on peripheral mononuclear white blood cells from 92 individuals between the ages of 24 and 45 from a community in and around Vancouver, Canada. When they looked at how methylation patterns at the promoters of nearly 14,500 human genes corresponded to individuals' gene expression profiles, blood composition, early-life socio-economic status and so on, the researchers found several factors that seemed to coincide with inter-individual methylation differences. These included the proportion of different white blood cell types present in the individuals' sample, their stress hormone output sex, age, and ethnicity as well as experiences such as childhood poverty and stress during adulthood. Even so, the DNA methylation differences detected often did not coincide with gene expression shifts at nearby genes, the researchers reported, "suggesting a more complex relationship than anticipated."

Another PNAS study looks at the bacterioplankton communities present in ocean samples collected in the Antarctic and the Arctic. By sequencing the V6 region of the 16S ribosomal RNA gene, Desert Research Institute investigators Alison Murray and colleagues from several centers around the world catalogued the bacteria found in surface or deep water bacterioplankton communities in the Southern and Arctic Oceans. The researchers then looked at how deep-water and shallow-water communities in the 20 Southern Ocean samples and the 24 Arctic Ocean samples tested compared with one another and with four-dozen samples collected at lower latitude ocean sites. "Our results suggest differences in environmental conditions at the poles and different selection mechanisms controlling surface and deep ocean community structure and diversity," the study's authors say. "Surface bacterioplankton may be subjected to more short-term variable conditions, whereas deep communities appear to be structured by longer water-mass residence times and connectivity through ocean circulation."

The Scan

Interfering With Invasive Mussels

The Chicago Tribune reports that researchers are studying whether RNA interference- or CRISPR-based approaches can combat invasive freshwater mussels.

Participation Analysis

A new study finds that women tend to participate less at scientific meetings but that some changes can lead to increased involvement, the Guardian reports.

Right Whales' Decline

A research study plans to use genetic analysis to gain insight into population decline among North American right whales, according to CBC.

Science Papers Tie Rare Mutations to Short Stature, Immunodeficiency; Present Single-Cell Transcriptomics Map

In Science this week: pair of mutations in one gene uncovered in brothers with short stature and immunodeficiency, and more.