Researchers at the Weill Cornell Medical College in New York and their colleagues this week present "an enhanced form of reduced representation bisulfite sequencing with extended genomic coverage, which [results] in greater capture of DNA methylation information of regions lying outside of traditional CpG islands." Applying this RRBS-based approach to primary human bone marrow specimens from patients with acute myelogeneous leukemia, the researchers found that "genetically distinct AML subtypes display diametrically opposed DNA methylation patterns," as they write in PLoS Genetics.
Over in PLoS One, investigators at the Cambridge Institute for Medical Research and elsewhere present a fluorescence-activated cell sorting-based approach to select for cell surface phenotype before performing non-lethal haploid genetic screens.
In the same journal, the Chinese University of Hong Kong's Joyce You et al. consider the cost-effectiveness of genotype-guided anticoagulation treatment in a Markov model designed to compare life-long economic and treatment outcomes of dabigatran, warfarin, and genotype-guided anticoagulation care in a hypothetical cohort of atrial fibrillation patients. "The likelihood of genotype-guided anticoagulation service to be accepted as cost-effective would increase if the quality of life on warfarin and dabigatran therapy are compatible and genotype-guided service achieves high TTR [patient-time in target range]," You et al. write.
In another paper appearing in PLoS One this week, researchers in China monitor the seroprevalence of human bocavirus species 1-4 in Beijing using a competition ELISA assay based on virus-like particles of HBoV1-4. "HBoV1 is more prevalent than HBoV2, HBoV3, and HBoV4 in the population we sampled in Beijing, China, suggesting that HBoV species may play differential roles in disease," the authors write.
And in PLoS Pathogens, an international team led by investigators at Ireland's University College Cork says multilocus sequence typing, or MLST, should replace serotyping-based classification for Salmonella.