This Week in PLoS

In PLoS One this week, investigators at Helicos BioSciences say that for RNA-seq analysis, "it is essential to understand how technical variation impacts the quality and interpretability of results, how potential errors could be introduced by the protocol, how the source of RNA affects transcript detection, and how all of these variations can impact the conclusions drawn." By running a variety of human RNA samples through fragmentation, fractionation, cDNA synthesis, and single- versus multiple-tag counting protocols, the Helicos team assessed the advantages and disadvantages of

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American scientists find themselves once again warning the Trump administration not to dismiss science, the New Yorker report.

A new study suggests CRISPR could be used to save coral reefs from dying off, Forbes reports.

Researchers have found that the i-motif shape of DNA previously observed in the lab also exists in human cells, and that it may serve a purpose.

In PNAS this week: a genomic, transcriptomic, and metabolomic analysis of the tea plant, Arabidopsis thaliana's adaptations to specific local environments, and more.