In the online edition of Nucleic Acids Research, investigators from the US and Korea outlined a computational strategy for dialing down off-target interactions identified when doing loss-of-function screens with small interfering RNAs. The "Deconvolution Analysis of RNAi screening data," or DecoRNAi, method can automatically assess and diminish rates of off-target effects, according to the study's authors, who validated the strategy using data from five siRNA screens.

Get the full story

This story is free
for registered users

Registering provides access to this and other free content.

Register now.

Already have an account?
Login Now.

Related Posts

In PNAS this week: genomic study of group B Streptococcus evolution, selection on the X chromosome in great apes, and more.

Changing the fat and fiber content of people's diets affects their gut microbiome, metabolome, and colon cancer risk, researchers say.

Broken links are found throughout academic publications, and some services are trying to combat such link decay.

Nick Stockton at Wired says that a pause in studying genome-editing tools should be used to find a path forward.