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In an advance online article published in Nucleic Acids Research this week, a trio of researchers at Harvard Medical School describes improved and complete enrichment, co-amplification at lower denaturation temperature PCR — or ice-COLD-PCR — which they say "enables rapid amplification and robust enrichment for low-abundance unknown DNA mutations." The team tested the efficacy of ice-COLD-PCR by enriching regions of TP53 in serially diluted mutant and wild-type DNA mixtures.

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