Researchers at the Broad Institute present "a process for transcriptome analysis of bacterial communities that accommodates both intact and fragmented starting RNA and combines efficient rRNA removal with strand-specific RNA-seq" in a Genome Biology paper published online in advance. The Broad team says its approach produces highly reproducible expression profiles that enrich up to 40-fold for non-rRNA transcripts and correlate "well with profiles representing undepleted total RNA."

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