In a paper published online in advance in Genome Biology this week, researchers at the University of Würzburg in Germany report their use of a differential RNA-seq approach with specific enrichment of primary transcripts to define the "transcriptome of purified elementary bodies and reticulate bodies of C.[Chlamydia] pneumoniae strain CWL-029." The Würzburg team mapped 565 transcriptional start sites of annotated genes and novel transcripts, and identified "a distinct transcription start site or an affiliation to an operon" for 862 out of 1074 annotated p

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