David Crotty writes that the June issue of CSH Protocols is full of ChIP-seq goodness. It includes an article from the National Heart, Lung and Blood Institute's Keiji Zhao that describes a method to prepare samples for ChIP-seq to study histone modification using the Illumina Genome Analyzer. "Step-by-step instructions are given for purification of human CD4+ T cells from lymphocytes and chromatin fragmentation using micrococcal nuclease (MNase) digestion, followed by chromatin immunoprecipitation (ChIP) and construction of a library for sequencing," Crotty says.
We Always Like Step-by-Step Instructions
Jun 02, 2009