Researchers at the University of Connecticut have developed a new method of multiple reaction monitoring mass-spec that can quantify targeted proteins. The team, led by Xudong Yao, calls it ultrathroughput MRM mass-spec. "It integrates the use of stable isotope dilution-multiple reaction monitoring mass spectrometry and peptide derivatization with inexpensive and commercial chemicals," the researchers write in their Analytical Chemistry study. Yao and his team say UMRM technology will have a "broad impact" on basic and applied biological and biomedical research. "Because of the flexibility in experimental design and reagent selection and the applicability of commercially available mass spectrometers, immediate adoption of the UMRM technology for targeted protein quantitation is expected," they write.
Apr 09, 2010