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Touch-Down Tips

At Bitesize Bio, Shoba Anantha gives some tips on using touch-down PCR. She says this "very useful technique for improving PCR amplification specificity is trickier that it might seem at first." To reduce non-specific priming, she suggests keeping the reactions cold until it's time for thermal cycling and then she says that a hot-start is the best way to go. She also suggests limiting the number of cycles. "Too many cycles will lead to appearance of non-specific bands in the gel," Anantha writes.

The Scan

Vaccine Update Recommended

A US Food and Drug Administration panel recommends booster vaccines be updated to target Omicron, CNBC reports.

US to Make More Vaccines for Monkeypox Available

The US is to make nearly 300,000 vaccine doses available in the coming weeks to stem the spread of human monkeypox virus, according to NPR.

Sentence Appealed

The Associated Press reports that Swedish prosecutors are appealing the sentence given to a surgeon once lauded for transplanting synthetic tracheas but then convicted of causing bodily harm.

Genome Biology Papers on COVID-19 Effector Genes, Virtual ChIP-seq, scDART

In Genome Biology this week: proposed COVID-19 effector genes, method to predict transcription factor binding patterns, and more.