At Bitesize Bio, James Hadfield gives an introduction to ChIP-seq for the uninitiated. Chromatin immunoprecipitation allows researchers to determine where protein-binding sites are located on DNA, and researchers coupled that approach to arrays, making ChIP-chip analyses. But, as Hadfield notes, "while this was a revolutionary approach, the technique is limited by the array technologies used." And so, chromatin immunoprecipitation was joined up with next-gen sequencing technologies. "ChIP-seq has been widely adopted since it was first reported in 2007. In fact, it has almost totally supplanted ChIP-chip, since it allows genome-wide analysis and does not have the limitations [of ChIP-chip]," he adds.
So That's ChIP-Seq
Nov 07, 2011