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Restriction Digests Made Easy

Aug 13, 2008

At Bitesize Bio, Rob offers tips for performing restriction digests. "Restriction digests are simple. But underestimate them at your peril," says Rob. Before running your screen, you should make a list of the available enzymes (avoid "unfriendly" ones) and identify restriction sites in both the insert and vector. He also offers a checklist of reasons the screen might have gone wrong.

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