At Bitesize Bio, Rob offers tips for performing restriction digests. "Restriction digests are simple. But underestimate them at your peril," says Rob. Before running your screen, you should make a list of the available enzymes (avoid "unfriendly" ones) and identify restriction sites in both the insert and vector. He also offers a checklist of reasons the screen might have gone wrong.
Restriction Digests Made Easy
Aug 13, 2008