Restriction Digests Made Easy | GenomeWeb

At Bitesize Bio, Rob offers tips for performing restriction digests. "Restriction digests are simple. But underestimate them at your peril," says Rob. Before running your screen, you should make a list of the available enzymes (avoid "unfriendly" ones) and identify restriction sites in both the insert and vector. He also offers a checklist of reasons the screen might have gone wrong.

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