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Perfect Precipitation Pellet (Say That 10 Times Fast)

To get a good DNA or RNA pellet through precipitation, you first need to choose between using ethanol and isopropanol. A post from Bitesize Bio a little while ago explains the choice — and when one approach is better than the other. However, blogger Suzanne Kennedy adds: “I use ethanol over isopropanol for most cases, but will use isopropanol if I need to make everything fit in one tube.” A follow-up post from John Mackay adds a tip on removing ethanol so you can be sure you got as much out as possible. After removing the 70 percent ethanol, he says to recap the tube and centrifuge it again. “Remove this liquid with a pipette and 200ul tip – you can get right alongside the pellet if visible. Leave the tube open as you move to the next sample,” he writes.

The Scan

Single-Cell Sequencing Points to Embryo Mosaicism

Mosaicism may affect preimplantation genetic tests for aneuploidy, a single-cell sequencing-based analysis of almost three dozen embryos in PLOS Genetics finds.

Rett Syndrome Mouse Model Study Points to RNA Editing Possibilities

Investigators targeted MECP2 in mutant mouse models of Rett syndrome, showing in PNAS that they could restore its expression and dial down symptoms.

Investigators Find Shared, Distinct Genetic Contributors to Childhood Hodgkin Lymphoma

An association study in JAMA Network Open uncovers risk variants within and beyond the human leukocyte antigen locus.

Transcriptomic, Epigenetic Study Appears to Explain Anti-Viral Effects of TB Vaccine

Researchers report in Science Advances on an interferon signature and long-term shifts in monocyte cell DNA methylation in Bacille Calmette-Guérin-vaccinated infant samples.