In a paper published online yesterday in Nature Methods, senior author Richard Gibbs and collaborators at Baylor and NimbleGen present a direct-selection technique for DNA sequences that does not require using PCR. The work took place using NimbleGen arrays and a 454 sequencer. "We combined these new developments to enable the efficient targeted capture and sequencing of specific DNA sequences from complex genomes to provide an alternative to PCR-based methods," the authors write.